Plant Tissue
Culture

 Plant Molecular
Biology

Seed   Maintenance and  storage

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• DNA extraction, using a protocol modified from Doyle et al  ;  1991

• Restriction endonuclease digestion of the DNA and ligation of adapters

• Amplification of the restriction fragments

• Gel analysis of the amplified fragment, using a polyacrylamide gel. Typically, 50 to 100 restriction fragments are complified in each AFLP reaction.

• artocapus heterophyllus  Lamk. (Jackfruit)

• Calamus  sp. (Rattan)

• Aegle marmelos  (L.) Corr. (Bel Fruit)

• Olea europaea  L. (Olive)

• Dorotis  sp.

Plant material (leaves)		Grind the plant material in a mortar		Transfer the ground tissue to eppendorf tube, add 1 ml. extraction buffer and precipitate DNA
		Visualise the DNA on the gel		Check DNA concentration on an agarose gel by electrophoresis;