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Micropropagation of plant material is commonly used for :
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Rapid,
large-scale, year round production of desired horticultural varieties
(e.g. many of popular miniature rose varieties are produced this
way).
-
Porduction of
plant species that are difficult to grow from seeds.
-
Production of
genetically uniform plant material (clones).
-
Development of
plant culture systems that are amenable to genetic transformation, e.g.
to introduce disease resistance, production of disease-free plant
material (e.g. potato microtubers)
Plant tissue culture media ingredients
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Micro-elements :
Fe, Mn, Co, Mo, B, Ni, Cl and Al
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Macro-elements :
Mg, p, S, N and K
-
Vitamins (B1,
nicotinic acid, pyridoxine-HCI etc.)
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Plant growth
regulators (auxins, cytokinins, etc.)
-
Carbon source
(sucrose)
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Organic nitrogen
(glycin, asparagine, etc.)
-
Agar (for solid
media)
Three are 3 main rooms :
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| Media preparation rooom |
Subculture transfer room |
Incubation room |
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