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Plant tissue culture is the science of plant propagation in vitro . The process includes many steps: stock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimation and growing of different lines. Micropropagation encompasses a range of tissue culture methods for propagation of plant species. In essence, tissue from a plant (explant) is isolated to create a sterile culture of that species in vitro . Once a culture is stabilized and growing well in vitro, multiplication of the tissue or regeneration of entire plants can be carried out. Shoots (tips, nodes or internodes) and leaf pieces are commonly used but cultures can be regenerated from many different tissues. Juvenile tissues generally respond best.
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